Substrates
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Substrates for hydrolytic Enzymes
The related pages list the phenolic portion of each substrate is abbreviated according to their constituent parts. For example the phenol SLPr is made from Syringaldehyde (S) by condensation with 1 propyl Lepidinium iodide (LPr), and VRA from Vanillin (V) and Rhodanin 3 Acetic acid (RA), etc. Further details on the characteristics of the chromophoric phenols are described by Aamlid et al. (Chem & Ind. 1989, 268 269). They all give intense colours at pH values > 7.0. The intensity of the colour can in some cases be increased by the use of an organic base. The lambda-max and extinction-max (extinction coefficient) of the phenols are those obtained when carbonate/bicarbonate buffer (approx pH 9.5) is used.
Also included are some MNP based substrates derived from the phenol 2-Methoxy 4 Nitrovinyl Phenol which have been described by Yuen et al., Clin. Chim. Acta, (1981) 112, 99; (1982) 124, 195; Ann. Clin. Biochem., (1984) 21, 295.
Key Benefits
- Highly coloured chromophores released following hydrolysis. They therefore form the basis of sensitive procedures
- Substrates have varying specificities. They therefore can be used in a variety of different analytical situations
- They are ideal for use as diagnostic indicators (in agars) for the presence of pathogenic bacteria
Substrates
Assay of:
