Mycoplasma
A Chromogenic Test for the rapid detection of Mycoplasma agalactiae and Mycoplasma bovis
Mycoplasma agalactiae and Mycoplasma bovis are closely related. M. agalactiae is the primary etiological agent of contagious agalactiae in sheep and goats. M. bovis is a frequently occurring bovine pathogen associated with mastitis, genital and ocular lesions and in calves and young cattle, pneumonia and arthritis.
The test involves using the novel PPR ester substrate. When a solution of this substrate is added to colonies of either M. bovis or M. agalactiae they become bright red within 30-60 min. In contrast strains of all other Mycoplasma species tested gave no colour change after 24 hours except for M. verecundum; this gave a diffuse red area around colonies after 3 hours.
The test may be conducted at room temperature or at 37°C (but not in a CO2 incubator). It is suitable for use on all mycoplasma agar media tested and identifies colonies directly on isolation media, e.g. plates inoculated with milk samples. Full details of the procedure are included with each kit which are available in three sizes depending on the number of samples to be analysed.
Mycoplasma Test Reagent has been developed for the rapid identification of M. agalactiae and M. bovis from cattle and sheep. The photograph shows M. agalactiae colonies after application of the test reagent to colonies grown on agar.
© PPR Diagnostics Ltd
The mycoplasma kit is shown on the left hand side and M. agalactiae colonies after application of the test reagent to colonies grown on agar are shown on the right.
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