NAG Kits (NAG Reagent kit)
Calibrant/Colorimetric Assay Method

NAG (N-Acetyl-β-D-glucosaminidase), hexosaminidase

NAG (HEX) is measured in urine as a well established, non-invasive marker of Acute Kindney Injury (AKI) or renal damage and disease. Renal disease causes a progressive deterioration in function which eventually results in renal failure which necessitates either renal dialysis or transplantation. The young are often affected and treatment may therefore be necessary for long periods of time. The kidney is also susceptible to secondary damage resulting from hypertension, diabetes, arthritis and urinary tract infection as well as being vulnerable to nephrotoxic drugs and environmental pollutants. However, the high reserve capacity of the kidney means that over 50% of active renal mass can be lost before there is a measurable change in renal function. Definitive diagnosis of AKI or renal disease is often based on the examination of renal biopsy samples or the measurement of serum creatinine which is modified after the disease is well established. However, this procedure is not without risk. There is therefore a need for, sensitive, non-invasive tests, as early detection improves the possibility of successful treatment, and at the same time reduces medical costs while improving the prospects of the patients. Such tests are also required for the assessment of new drugs for potential nephrotoxicity, and monitoring for the effect of environmental or occupational toxins (for example, heavy metals or solvents).

Hexosaminidase (NAG) isoenzymes are present in different organs and tissues. The acidic A and basic B forms are present in the liver, kidney and spleen. However, in serum the major form is the As-form but two additional intermediate forms I1 and I2 are also present. The latter resembles the P-form which is found in the serum of pregnant women. Hexosaminidase is assayed in Laboratories monitoring genetic disease in suspected cases of Tay-Sach disease and Sandhoff’s disease. The A-form of HEX is absent from Tay-Sach disease which is most commonly found in Ashkenasi Jews.

Keywords: NAG Kit, NAG Reagent Kit, NAG Assay, N-Acetyl-β-D-glucosaminidase, NAG Colorimetric Assay, NAG Chromogenic Assay Kit, Acute Kindney Injury (AKI), Chronic Kidney Disease (CKD), renal failure, renal damage, renal biomarker, early biomarker for AKI or kidney failure, Urinary (NAG) Assay kit, MNP-GlcNAc substrate, NAG enzyme, early marker of renal failure.

Key Benefits

• Well established early warning of acute kidney injury (AKI) and renal damage
• Colorimetric procedure adaptable to any laboratory
• Can be used in conjunction with heat treatment or ion-exchange chromatography in the quantitation of the A and B-forms of hexosaminidase
• 3 Kits available in various sizes to suit type of investigation

References

Method Sheets

Method sheets are available for the manual assay of N-acetyl-ß-D-glucosaminidase in urine or if required serum are supplied. In addition the assay procedure can be used to detect the isoenzymes of NAG particularly the A and B forms. If the assay is to be carried out in the routine laboratory, method sheets for most of the commonly used autoanalysers are available on request.

View method sheet for NAG Calibrant procedure

Method sheet immediately available

Bayer Diagnostics

• Axon
• R.A. 1000

Hitachi

• Hitachi 704 as well as Hitachi 705
• Hitachi 717
• Hitachi 917
• Hitachi Modular P800

International Laboratories Monarch

• Multistat III
• I.L. Monarch

Roche Products

• Cobas Bio
• Cobas Fara II
• Cobas Mira S

Other

• Beckman
• Olympus AU400
• Ilab 900
• Konelab 6.5.4
• A procedure is available which enables the NAG kit to be used in conjunction with 96-well plate readers.

We are in discussions with the above and other manufacturers to provide methodology for the latest additions to their range. Please email for details if your instrument is not listed.

MSDS sheet 

Click here for MSDS for NAG substrate.

NAG kits (Calibrant method)

Ordering Information 

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