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Mycoplasma Detection kit

 

A Chromogenic Test for the rapid detection of Mycoplasma agalactiae and Mycoplasma bovis in veterinary diseases 


Contents*Product Code
1 kit - 25 tests  1103A
1 kit - 50 tests 1103B

* Contains sufficient reagent to test A. 25 or B. 50 agar plates.

 

Introduction

Mycoplasma agalactiae and Mycoplasma bovis are closely related. M. agalactiae is the primary etiological agent of contagious agalactiae in sheep and goats. M. bovis is a frequently occurring bovine pathogen associated with mastitis, genital and ocular lesions and in calves and young cattle, pneumonia and arthritis.


Application

This is a qualitative test involving PPR substrate giving a rapid color change directly on the culture media to detect the presence of Mycoplasma. Mycoplasma Test Reagent has been developed for the rapid identification of M. agalactiae and M. bovis from cattle and sheep. PPR Mycoplasma Detection Kit is one of the simplest and cheapest detection methods available today.

 

Key Benefits 

Very Fast: Detection time within 30-60 minutes

Easy: Extremely simple test and takes just a single step 

High Specificity: Test is highly specific for detection of Mycoplasma agalactiae and Mycoplasma bovis in 1 hour. M. verecundum appears diffused red in 3 hours

Versatility: Suitable for all Mycoplasma agar media

At Room Temperature


Product Description

Time taken: 30 to 60 minutes

Sample requirement: Mycoplasma grown on any suitable media, methanol

Contains: PPR Rapichrome™ Mycoplasma reagent (to be reconstituted in Ringer’s HEPES buffer) sufficient to test 25 or 50 agar plates.

 

Kit Contents

1. PPR Rapichrome™ Mycoplasma test reagent, freeze dried x 5 vials (1103A) or 10 vial (1103B)

2. Ringer’s HEPES buffer 5 mL (1103A) or 10 mL (1103B) (Methanol not provided)

3. Instructional sheet included

 

Mycoplasma Detected

Mycoplasma agalactiae 

Mycoplasma bovis

Mycoplasma verecundum (in 3 hours) 

Image © PPR Diagnostics Ltd

kitmycoplasma

Image © PPR Diagnostics Ltd

The mycoplasma kit is shown on the left hand side and M. agalactiae colonies after application of the test reagent to colonies grown on agar are shown on the right.

 

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